I recommend to play with parameters to get a nice detection result. ROIs will have names in the format of ind(detection# in Results table)_ch(#channel)_sl(#slice)_fr(#frame).Īfter pressing OK plugin runs and add ovals/rectangles in overlay on top of detected particles.Īlso it will provide you Results table containing particles’ coordinates (see below) and Summary table. If you choose Add to ROI Manager option (“All particles”), plugin will add detected rectangular ROIs around particles to ROI Manager. As its name suggests, this procedure requires computational power and can be slow, especially on big images. If “ Segment larger particles (slow)?” box is checked, plugin will try to further split large particles into a smaller dots, if it is possible. If you check “ Include larger particles?” box, it will also try to quantify bigger spots. The “ROI shape” option allows you to choose, if you want your detection to be displayed as “Ovals” or “Rectangles”.īy default, plugin looks only for particles of specified size. Checkbox “ Preview…” allows to see detection on the current picture and simplifies this task. To have good detection usually you need to play with parameters and see how it goes. Specify estimated particles size and intensity threshold (particle brightness) and press OK. If the image is not multi-channel image, the following dialog window will appear: Launch the detection by choosing Plugins › ComDet › Detect Particles. Plugin auto-detects the number of channels and if it is more than one then the plugin will automatically switch to the second mode (see below).
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